Reductive activation of mitomycin C and mitomycin C metabolites catalyzed by NADPH-cytochrome P-450 reductase and xanthine oxidase.
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چکیده
منابع مشابه
Activation of mitomycin C by NADPH:cytochrome P-450 reductase.
Mitomycin C is an alkylating agent used in cancer chemotherapy that shows some specificity towards hypoxic cells. The therapeutic effects of this compound are thought to result from its metabolic activation by enzymes such as NADPH:cytochrome P-450 reductase. In a previous report we described a Chinese hamster ovary cell line resistant to mitomycin C, which had a decreased NADPH:cytochrome P-45...
متن کاملActivation of Mitomycin C by NADPH:Cytochrome P-450 ReducÃ-ase1
Mitomycin C is an alkylating agent used in cancer chemotherapy that shows some specificity towards hypoxic cells. The therapeutic effects of this compound are thought to result from its metabolic activation by enzymes such as NADPH:cytochrome P-450 reducÃ-ase.In a previous report we described a Chinese hamster ovary cell line resistant to mitomycin C, which had a decreased NADPH:cytochrome P-45...
متن کاملThe Reactions of Mitomycin C with Dithiols I. Reductive Activation
We report that the clinically used antitumor drug mitomycin C is reductively activated in vitro by simple thiols; a mechanism for this activation is proposed based on kinetic data and the identification of mitosene metabolites. The biological implications of these findings are discussed. Introduction Mitomycin C (MC, 1) 1 is a natural antitumour antibiotic used in anticancer therapy 2 that requ...
متن کاملNADPH-cytochrome c reductase and its role in microsomal cytochrome P-450-dependent reactions.
The study of liver microsomal electron transport and drug metabolism activities involving NADPH-cytochrome c reductase has been performed using immunochem ical techniq ues ( I -4). This approach has been particularly valuable in establishing the role of this flavoprotein in the reduction of microsomal cytochrome P-450 and, thus, in the metabolism of drugs (2, 3). The existence of NADPH-cytochro...
متن کاملEnhancement of xanthine dehydrogenase mediated mitomycin C metabolism by dicumarol.
These studies examined the effect of dicumarol on xanthine dehydrogenase (XDH), an enzyme recently shown to bioreduce mitomycin C. Dicumarol, which has previously been shown to inhibit xanthine oxidase (XO), inhibited both XDH and XO mediated conversion of xanthine to uric acid but potentiated the metabolism of mitomycin C by XDH and XO. Formation of 2,7-diaminomitosene following mitomycin C bi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)43551-4